LOCUS       sequence-134633-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes chloramphenicol resistance gene for 
            positive selection and a toxin gene (relE) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-242
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     547..566
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(633..735)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 agtactgttg tattcattaa gcatctgccg acatggaagc catcacaaac ggcatgatga
       61 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      121 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      181 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
      241 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
      301 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
      361 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgtaattcc
      421 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
      481 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
      541 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
      601 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
      661 gacaactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
      721 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
      781 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg taggcgcgcc
//
