LOCUS       sequence-134638-         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_PBX2_1
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..17
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             24..44
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagacgatga cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc
       61 cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg
      121 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
      181 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
      241 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
      301 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
      361 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
      421 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
      481 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
      541 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgac
//
