LOCUS       sequence-134640-         266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 266)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ZNF146_1
FEATURES             Location/Qualifiers
     source          1..266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..38
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgacgataa gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag
       61 ccgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga
      121 tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc
      181 acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg
      241 ccacccggct gaagagaacc gccaga
//
