LOCUS       sequence-134642-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ZNF146_2
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        109..357
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     109..129
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     280..299
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        378..453
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtga gggcctattt
      121 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt
      181 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
      241 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
      301 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgcc
      361 actttcaagg ctttcatgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      421 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct agaaatagca
      481 agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac aaatggctct
      541 agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      601 ccgcccattg acgtcaatag taacgcc
//
