LOCUS       sequence-134643-         309 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 309)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ZNF146_2
FEATURES             Location/Qualifiers
     source          1..309
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..50
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             57..77
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acggagacta caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa
       61 agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg
      121 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
      181 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
      241 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
      301 ccagaagaa
//
