LOCUS       sequence-134644-         533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 533)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ZNF3_1
FEATURES             Location/Qualifiers
     source          1..533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             58..78
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gacggagact acaaggatca tgatattgat tacaaagacg atgacgataa gatggcccca
       61 aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtacagcatc
      121 ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
      181 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
      241 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
      301 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
      361 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
      421 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
      481 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cag
//
