LOCUS       sequence-134645-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ZNF3_2
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..39
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatgacgata agatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca
       61 gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
      121 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
      181 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
      241 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
      301 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
      361 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
      421 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
      481 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
      541 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
      601 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
      661 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
      721 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga
//
