LOCUS       sequence-134653-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human DNMT3B along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_DNMT3B
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..53
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             60..80
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acgacggaga ctacaaggat catgatattg attacaaaga cgatgacgat aagatggccc
       61 caaagaagaa gcggaaggtc ggtatccacg gagtcccagc agccgacaag aagtacagca
      121 tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
      181 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
      241 tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa
      301 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
      361 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
      421 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
      481 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
      541 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
      601 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
      661 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
      721 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
//
