LOCUS       sequence-134657-        1422 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV 
            cleavage site-linked fusion proteins with C-terminal 
            6His-tag/Strep-tag II/HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Bokhove M, Sadat Al Hosseini H, Saito T, Dioguardi E, 
            Gegenschatz-Schmid K, Nishimura K, Raj I, de Sanctis D, Han L, 
            Jovine L
  TITLE     Easy mammalian expression and crystallography of maltose-binding 
            protein-fused human proteins.
  JOURNAL   J Struct Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi:
            10.1016/j.jsb.2016.01.016.
  PUBMED    26850170
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi: 
            10.1016/j.jsb.2016.01.016."
COMMENT     Sequence Label: pHLmMBP-10
FEATURES             Location/Qualifiers
     source          1..1422
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             94..117
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1252..1272
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1303..1320
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1348..1371
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1390..1416
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggggatcc ttcccagccc tgggatgcct gcgctgctct ccctcgtgag ccttctctcc
       61 gtgctgctga tgggttgcgt agctgaaacc ggtcaccatc accatcacca tcaccataaa
      121 accgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct
      181 gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatcccgat
      241 aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc
      301 tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg
      361 gccgcagcgt tccaggacaa gctgtatccg tttacctggg atgccgtgcg ctacaacggc
      421 aagctgattg cttacccgat cgctgttgaa gcgttgtccc tgatttataa caaggatctg
      481 ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg
      541 aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt
      601 gctgctgacg ggggttatgc gttcaagtat gcagccggaa agtacgacat taaagacgtg
      661 ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac
      721 aaacacatga atgcagacac cgattactcc atcgcagaac atgcctttaa tcatggcgaa
      781 acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag cgcagtgaat
      841 tatggtgtaa cggtgctgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg
      901 ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa
      961 aactacctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc
     1021 gtagcgctga agtcttacga ggaagagttg gtgaaagatc cacgtgttgc cgccacaatg
     1081 gaaaacgccc agaaaggtga aatcatgccc aacatcccgc agatgtccgc tttctggtat
     1141 gccgtgcgca ctgcggtgat taacgccgcc agcggtcgtc agactgtcga tgcagccctg
     1201 gcagccgcgc agactaatat caccaagggc ggagggagcg gcggagggag cgaaaacctg
     1261 tattttcaga gcgcggccgc cgcgagctcg gctagcctcg agcaccatca ccatcaccat
     1321 tgataaacgc gtttaattaa ccccgggtgg agccaccccc agttcgagaa gtgataagat
     1381 gatcaaggat atccctacga cgtgcccgac tacgcctgat aa
//
