LOCUS       sequence-134660-         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ZNF219_1
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagaagcgga aggtcggtat ccacggagtc ccagcagccg acaagaagta cagcatcggc
       61 ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
      121 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
      181 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
      241 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
      301 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
      361 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
      421 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
      481 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
      541 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg aca
//
