LOCUS       sequence-134661-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ZNF219_2
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        102..350
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     102..122
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     273..292
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        371..446
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
       61 tttacggttc ctggcctttt gctggccttt tgctcacatg tgagggccta tttcccatga
      121 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
      181 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      241 agnttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      301 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gctaagctaa
      361 tcgcccctga gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      421 ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga gctagaaata gcaagttaaa
      481 ataaggctag tccgttttta gcgcgtgcgc caattctgca gacaaatggc tctagaggta
      541 cccgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      601 ttgacgtcaa tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      661 cggtaaactg cccacttgg
//
