LOCUS       sequence-134662-         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ZNF219_2
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             56..76
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
       61 gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg
      121 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
      181 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
      241 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
      301 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
      361 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
      421 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
      481 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
      541 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
      601 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatcca
//
