LOCUS       sequence-134664-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_SSRP1_1
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag
       61 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag
      121 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
      181 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
      241 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
      301 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
      361 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
      421 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
      481 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
      541 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
      601 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aa
//
