LOCUS       sequence-134684-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High efficiency Streptomyces genome editing by CRISPR/Cas9 system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Huang H, Zheng G, Jiang W, Hu H, Lu Y
  TITLE     One-step high-efficiency CRISPR/Cas9-mediated genome editing in 
            Streptomyces.
  JOURNAL   Acta Biochim Biophys Sin (Shanghai). 2015 Apr;47(4):231-43. doi: 
            10.1093/abbs/gmv007. Epub 2015 Mar 3.
  PUBMED    25739462
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/abbs/gmv007"; journalName: "Acta Biochim Biophys Sin 
            (Shanghai)"; date: "2015-04"; volume: "47"; issue: "4"; pages: 
            "231-43"
COMMENT     Sequence Label: pKCcas9dO
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
       61 caccgagtcg gtgctttttt tgagatctga attccaccaa gccggagtcg gtggtgggcg
      121 aggagatcct gacccggcac ttcccggccg gctccggtga acccatggtc gtcatcgcca
      181 agggcgcctc ggcggaccag gtgcacgcag ctctggagac cgtgcccggc gtcatcgagg
      241 tcgcaccacc acaggtgaag gacggcctcg cctatgtcga ggcgacgctc ggcgccgggg
      301 cggactcgcc ggcggccatg cgttcggtca ccgcggcccg cgagacactc gcccggctgg
      361 acggtgcgca ggcacgggtg ggcggcagca gcgccgtcgt gcacgacatg cgggaggcgt
      421 ccagccgcga ccgcggtctg atcattccgg tgatcctcgc cgtggtgttc tgcatcctcg
      481 cgctgctgct gcgagcactg gtggcgccgc tgctgctgat cgcgagcgtg gtgctgtcct
      541 tcttcaccgc gctcggcctc gccaccctct tcttcaacca cgtcttcgac ttcgcgggag
      601 ccgattcggc cttccccctg tgggtcttcg tcttcctggt cgccctgggc gtcgactaca
      661 acatcttcct cgtcacccgg atcaaggagg agagcgaccg gctcggcacc cggcagggcg
      721 cgctcaaggg cctcacctcg accggtggcg tgatcaccgc ggccgggctg gtgctggccg
      781 ggaccttcgc cgcgctggcc acgctgccgc tggtcttcat cgccgagctc ggattcacgg
      841 tcgcggtggg cgtcctgctc gacaccatga tcgtgcgatc ggtcctggtc accgcgctca
      901 ctctcgatgt cgg
//
