LOCUS       sequence-134685-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human APE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Schuermann D, Scheidegger SP, Weber AR, Bjoras M, Leumann CJ, Schar 
            P
  TITLE     3CAPS - a structural AP-site analogue as a tool to investigate DNA 
            base excision repair.
  JOURNAL   Nucleic Acids Res. 2016 Jan 4. pii: gkv1520.
  PUBMED    26733580
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jan 4. pii: gkv1520."
COMMENT     Sequence Label: pET28HIS-hAPE1
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(49..66)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(67..84)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agctattagt gatgatgatg
       61 atgatggctg ccgcgcggca ccaggccgct gctaagcttg tcgaccagtg ctaggtatag
      121 ggtgatagga cagtgatcac tgccgagggc cttggaacgg atcttgctgt cacacaatgc
      181 aggtaacaga gagtgggaca acaaaaagta atcaaggcgc caaccaacat tcttggatcg
      241 agcattcatc atataagtcc aaaaggtgta ggcatagggt gtgttggggt agaggtgcct
      301 aaagctgtca gccagtggca cagcctgcag taattccccg aagccttggc gctcttgtgg
      361 cgtgaagcca gcattctttt tgttcccctt ggggttgcga aggtcaattt cttcatgtgc
      421 cacattgagg tctccacaca gcacaagggg ctttcgggaa gccaggccct tcaggaactt
      481 gcgaaaggct tcatcccagc gctgccggta ctccagtcgt accagacctc ggcctgcatt
      541 aggtacatat gctgttacca gcacaaacga gtcaaattca gccacaatca cccggccttc
      601 ctgatcatgc tcctcatcgc ctatgccgta agaaactttg agtgggcact ggcgggaaag
      661 caggcccacg ccactgtacc cttccttgtc cgaaggagct gaccagtatt gatgagagag
      721 tccaggcagc tcctgaagtt cagctggtag tttgttctct gaacatttgg tctcttgaag
      781 gcacagtata tct
//
