LOCUS       sequence-134687-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion 
            protein protein. Contains the catalytic domain of mouse Dnmt3a and 
            the C-terminal part of mouse Dnmt3L connected by 27 aa linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Siddique AN, Nunna S, Rajavelu A, Zhang Y, Jurkowska RZ, Reinhardt 
            R, Rots MG, Ragozin S, Jurkowski TP, Jeltsch A
  TITLE     Targeted methylation and gene silencing of VEGF-A in human cells by 
            using a designed Dnmt3a-Dnmt3L single-chain fusion protein with 
            increased DNA methylation activity.
  JOURNAL   J Mol Biol. 2013 Feb 8;425(3):479-91. doi: 
            10.1016/j.jmb.2012.11.038. Epub 2012 Dec 4.
  PUBMED    23220192
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2012.11.038"; journalName: "J Mol Biol"; date: 
            "2013-02-8- 8"; volume: "425"; issue: "3"; pages: "479-91"
COMMENT     Sequence Label: pET28-Dnmt3a3L-sc27
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag ttaaagagga agtgagtttt
       61 gagaaaaata cttgaagtac tctctcagcg ggagaaggca gttcttcacc aggaggtcaa
      121 ctttcggggc gtccagcttg ctcctgcttc tgacttgggc ttgcagatac tcttcttcct
      181 ttggggtcag gggcgcatgc ttgctcttca gccctggaat gttgctccac acccgcatag
      241 cattctggta gtccctgcca cggacatcct ggagggtcac agcctctgtc tgaaggaagc
      301 gggtagttgt ctcttggtca tcctcagtca gcagcagatt gtccatgaat atccagaaga
      361 agggccgctg actctcctgg cgaggcagcg catactgcag gatccggtgg aactggaaca
      421 tgtaccagcc gggacagcga tcacaggagc tgcctagggg ctgcgtcgag ccgtacacca
      481 ggtcaaaggg gccccatttc tccacgtctc tcctcacgac atttgtgaca tcttccacgt
      541 acttcagcgt tcctccccca gaaccagaac cgctttctaa aaagcccaaa ctctttagta
      601 ctttatcaat atttctaaaa aggctcagta cccgcactgg ctgtctcttc catgcagaca
      661 ctgtcttgta tatctccata gggcccatat ggctgccgcg caagcttaac ggcaccaggc
      721 cgctgctgaa gctcggcccg cggctgttag cgttactatt gccgctagac acacaagcaa
      781 aatattcctt cagcggagcg aagaggtggc ggatgaccgg cacgctccac gatcggccca
      841 gcagtctctg cctcgccaag cggctcatgt tggagacgtc tgtgtagtgg acggggaagc
      901 caaacaccct tt
//
