LOCUS       sequence-134689-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-fluorescent surrogate reporter vector for in vitro functional 
            assay of programable DNA nuclease and enrichment of genetically 
            edited cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Zhou Y, Liu Y, Hussmann D, Brogger P, Al-Saaidi RA, Tan S, Lin L, 
            Petersen TS, Zhou GQ, Bross P, Aagaard L, Klein T, Ronn SG, Pedersen
            HD, Bolund L, Nielsen AL, Sorensen CB, Luo Y
  TITLE     Enhanced genome editing in mammalian cells with a modified 
            dual-fluorescent surrogate system.
  JOURNAL   Cell Mol Life Sci. 2016 Jan 11.
  PUBMED    26755436
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2016 Jan 11."
COMMENT     Sequence Label: P119 C-Check
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      548..557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aatttttcgc aacgggtttg ccgccagaac acaggtacac atattgacca aatcagggta
       61 attttgcatt tgtaatttta aaaaatgctt tcttctttta atatactttt ttgtttatct
      121 tatttctaat actttcccta atctctttct ttcagggcaa taatgataca atgtatcatg
      181 cctctttgca ccattctaaa gaataacagt gataatttct gggttaaggc aatagccgat
      241 tagttcgtac gggccggcca tgcatctttt ggagaatatt tctgcatata aatatttctg
      301 catataaatt gtaactgatg taagaggttt catattgcta atagcagcta caatccagct
      361 accattctgc ttttatttta tggttgggat aaggctggat tattctgagt ccaagctagg
      421 cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg ggcaacgtgc
      481 tggtctgtgt gctggcccat cactttggca aagcacgtga gatctgaatt ctgacacacc
      541 cccgggcgcc accatggcct ctttgctgaa aaagaccatg cccttcagga ccaccatcga
      601 gggcaccgtg aacggccact acttcaagtg caccggcaag ggcgagggca accccctgga
      661 gggcacccag gagatgaaga tcgaggtgat cgagggcggc cccctgccct tcgccttcca
      721 catcctgtcc acctcctgca tgtacggctc caaggccttc atcaagtacg tgtccggcat
      781 ccccgactac ttcaagcagt ccctccccga gggcttcacc tgggagcgca ccaccaccta
      841 cga
//
