LOCUS       sequence-134692-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of ChrimsonR-tdTomato under the Syn 
            promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA 
            signal. TdTomato is a codon diversified version..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..104
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    175..208
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      230..239
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt cgcgaacctc
       61 ttcgagggac ataacttcgt ataatgtatg ctatacgaag ttatgggtcg atggtgagat
      121 ctggactaga gggtcgatgg tgatgcttgg atcaaaatag gaagaatccc aatgataact
      181 tcgtataaag tatcctatac gaagttatac taggtaccta ggccgcgccg ccaccatggc
      241 tgagctgatc agcagcgcca ccagatctct gtttgccgcc ggaggcatca acccttggcc
      301 taacccctac caccacgagg acatgggctg tggaggaatg acacctacag gcgagtgctt
      361 cagcaccgag tggtggtgtg acccttctta cggactgagc gacgccggat acggatattg
      421 cttcgtggag gccacaggcg gctacctggt cgtgggagtg gagaagaagc aggcttggct
      481 gcacagcaga ggcacaccag gagaaaagat cggcgcccag gtctgccagt ggattgcttt
      541 cagcatcgcc atcgccctgc tgacattcta cggcttcagc gcctggaagg ccacttgcgg
      601 ttgggaggag gtctacgtct gttgcgtcga ggtgctgttc gtgaccctgg agatcttcaa
      661 ggagttcagc agccccgcca cagtgtacct gtctaccggc aaccacgcct attgcctgcg
      721 ctacttcgag tggctgctgt cttgccccgt gatcctgatc agactgagca acctgagcgg
      781 cctgaagaac gactacagca agcggaccat gggcctgatc gtgtcttgcg tgggaatgat
      841 cgtgttcggc atggccgcag gactggctac cgattggctc aagtggctgc tgtatatcgt
      901 gtcttgcatc tacggcggct acatgtactt ccaggccgcc aagtgctacg tggaagccaa
      961 ccacagcgtg cctaaaggcc attgccgcat ggtcgtgaag ctgatggcct acgcttactt
     1021 cgcctcttgg g
//
