LOCUS       sequence-134694-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of Chronos-GFP under the EF1-alpha promoter,
            in floxed/reversed (Cre-dependent) manner. Using bGHpA signal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pAAV-EF1-alpha-FLEX-rc[Chronos-GFP]
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcaaat gaacttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcactg ctactaccgg
      301 tgccgccact cctccctcct cctcctcgtc gacgaaggtc tcgatctcca tgttctcgcc
      361 ggccacgttg atggtggttg tcttccggat gtcgccgtgg atcaggatgt gctcgtggat
      421 cttcactctc aggtggtggc ccaggaagcc ccaagtgttc ttgctgatca gatccaggat
      481 caggtggccg attccgctgg tgtaaggtgt gatcaggccc agtccctcgt gtccggcgat
      541 gaacatcacg gggaacatca gccaagagca gaagaagacg taggccatga tcttgcaaat
      601 cttccggcag acgcctttgg gcagggtgtg gtagctctcg atatacacct tggcgatctg
      661 gaagaagcaa gtcacgccgt agaacaggcc gatcatgaag aacaggatct tcagggggcc
      721 ctttgtaaag gcggctgtga tcccccacac gatgttgccg atgtcggtca ccaggatggt
      781 catggtccgc ttgctgtact cttcgtgcag tccggtcagg ttgctcaggt ggatcaggat
      841 cacggggcaa gtcaggagcc acatgctgta ccgcagccaa atcacggctc ctccgttggt
      901 ctggtacact gtggctgggc tgtcgacctc gtggaacagc tcgatgaagc acttgacgag
      961 ctcgatcacg cagacgtaga cctcctccca gccggttgtg gccttgaagc tgtgccaacc
     1021 gtagaagatg agctgcacga tagccacgat cacg
//
