LOCUS       sequence-134700-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains 8xLexA-binding elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Fujita T, Fujii H
  TITLE     Direct identification of insulator components by insertional 
            chromatin immunoprecipitation.
  JOURNAL   PLoS One. 2011;6(10):e26109. doi: 10.1371/journal.pone.0026109. Epub
            2011 Oct 17.
  PUBMED    22043306
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(10):e26109. doi"; pages: " 
            10.1371/journal.pone.0026109. Epub 2011 Oct 17"
COMMENT     Sequence Label: 8xLexA-binding elements/pMD20
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..113
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(151..173)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     273..292
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     485..507
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     499..516
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     500..516
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(817..834)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(852..868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(852..868)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(865..887)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    876..892
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(900..906,907..924,925..930))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    945..966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
       61 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
      121 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
      181 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
      241 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
      301 gcactatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcagggc
      361 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      421 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      481 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga
      541 tccgattgtg tcgactctat tcagcggatc tcgagcgtac tgtacatata accactggtt
      601 ttatatacag cagtactgta catataacca ctggttttat atacagcagt cgaggcgtac
      661 tgtacatata accactggtt ttatatacag cagtactgta catataacca ctggttttat
      721 atacagcagt cgaggtacct atcgatagag aatctagatt aatccatatg actagtagat
      781 cctctagagt cgacctgcag gcatgcaagc tttcccctat agtgtcacct aaatagcttg
      841 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      901 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      961 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1021 cattaatgaa tcggccaacg cgcgggg
//
