LOCUS       sequence-134705-        1377 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes kanamycin resistance gene for 
            positive selection and toxin gene (chpB) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-238
FEATURES             Location/Qualifiers
     source          1..1377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      130..216
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      308..335
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     regulatory      389..398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        538..656
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      1090..1336
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 tcatgctgga gttcttcgcc caccccagct tcaaaagcga tcctccgcgg cccaaagtgc
       61 cacctgcatc gatggccccc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
      121 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      181 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
      241 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa
      301 attaagcaga aggccatcct gacggatggc ctttttgcgt ggccagtgcc aagcttgcat
      361 gcctgcagct gtccggaact agtcgaggcc atggtggcct cctgatgtcg tcaacacggc
      421 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
      481 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
      541 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
      601 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
      661 aattcagcag gatcaccata tggtaaagaa aagtgaattt gaacggggag acattgtgct
      721 ggttggcttt gatccagcaa gcggccatga acagcaaggt gctggtcgac ctgcgcttgt
      781 gctctccgtt caagccttta atcaactggg aatgacgctg gtggccccca ttacgcaggg
      841 cggaaatttt gcccgttatg ccggatttag cgttccttta cattgcgaag aaggcgatgt
      901 gcacggcgtg gtgctggtga atcaggtgcg gatgatggat ctacacgccc ggctggcaaa
      961 gcgtattggt ctggctgcgg atgaggtggt ggaagaggcg ttattacgct tgcaggcggt
     1021 ggtggaataa ggatccgcga attctcatgt ttgacagctt atcactgatc agtgaattaa
     1081 tggcgatgac gcatcctcac gataatatcc gggtaggcgc aatcactttc gtctctactc
     1141 cgttacaaag cgaggctggg tatttcccgg cctttctgtt atccgaaatc cactgaaagc
     1201 acagcggctg gctgaggaga taaataataa acgaggggct gtatgcacaa agcatcttct
     1261 gttgagttaa gaacgagtat cgagatggca catagccttg ctcaaattgg aatcaggttt
     1321 gtgccaatac cagtagaaac agacgaagaa gctagctttg cactggatgg ggacgtc
//
