LOCUS       sequence-134707-        1487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes kanamycin resistance gene for 
            positive selection and toxin gene (yhaV) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-239
FEATURES             Location/Qualifiers
     source          1..1487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      130..216
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      308..335
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     regulatory      389..398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        538..656
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      1204..1450
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 tcatgctgga gttcttcgcc caccccagct tcaaaagcga tcctccgcgg cccaaagtgc
       61 cacctgcatc gatggccccc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
      121 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      181 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
      241 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa
      301 attaagcaga aggccatcct gacggatggc ctttttgcgt ggccagtgcc aagcttgcat
      361 gcctgcagct gtccggaact agtcgaggcc atggtggcct cctgatgtcg tcaacacggc
      421 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
      481 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
      541 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
      601 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
      661 aattcagcag gatcaccata tggattttcc acaaagggtt aatggttggg cgctatatgc
      721 tcatccctgt tttcaggaaa cctacgacgc tttagttgcc gaagtcgaga cattaaaggg
      781 aaaagatcct gaaaattatc agagaaaagc cgccacaaag ttattggcgg tagtccataa
      841 agtgattgag gagcatatca cggtcaatcc atcatcaccg gcattccgtc atggcaagtc
      901 gttaggctct gggaaaaata aagactggtc acgggtaaaa tttggtgctg gtcgttatcg
      961 tctcttcttt cgttatagtg aaaaagagaa agtcatcatt ctgggatgga tgaacgatga
     1021 aaacactctg cgcacctacg gtaaaaaaac agatgcctat accgtattca gcaaaatgtt
     1081 aaaaagagga catcctcctg ccgactggga aaccctcacc cgagaaacag aagaaaccca
     1141 ttgaggatcc gcgaattctc atgtttgaca gcttatcact gatcagtgaa ttaatggcga
     1201 tgacgcatcc tcacgataat atccgggtag gcgcaatcac tttcgtctct actccgttac
     1261 aaagcgaggc tgggtatttc ccggcctttc tgttatccga aatccactga aagcacagcg
     1321 gctggctgag gagataaata ataaacgagg ggctgtatgc acaaagcatc ttctgttgag
     1381 ttaagaacga gtatcgagat ggcacatagc cttgctcaaa ttggaatcag gtttgtgcca
     1441 ataccagtag aaacagacga agaagctagc tttgcactgg atgggga
//
