LOCUS       sequence-134709-        1323 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes kanamycin resistance gene for 
            positive selection and toxin gene (mqsR) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-241
FEATURES             Location/Qualifiers
     source          1..1323
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      130..216
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      308..335
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     regulatory      389..398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        538..656
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      1036..1282
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 tcatgctgga gttcttcgcc caccccagct tcaaaagcga tcctccgcgg cccaaagtgc
       61 cacctgcatc gatggccccc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
      121 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      181 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
      241 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa
      301 attaagcaga aggccatcct gacggatggc ctttttgcgt ggccagtgcc aagcttgcat
      361 gcctgcagct gtccggaact agtcgaggcc atggtggcct cctgatgtcg tcaacacggc
      421 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
      481 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
      541 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
      601 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
      661 aattcagcag gatcaccata tggaaaaacg cacaccacat acacgtttga gtcaggttaa
      721 aaaacttgtc aatgccgggc aagttcgtac aacacgtagt gccctgttaa atgcagatga
      781 gttaggtttg gattttgatg gtatgtgtaa tgttatcatt ggattatcag agagcgactt
      841 ttataaaagc atgaccacct actctgatca tactatctgg caggatgttt acagacccag
      901 gcttgttaca ggccaggttt atcttaaaat tacggtaatt catgacgtac tgatcgtctc
      961 gtttaaggag aagtaaggat ccgcgaattc tcatgtttga cagcttatca ctgatcagtg
     1021 aattaatggc gatgacgcat cctcacgata atatccgggt aggcgcaatc actttcgtct
     1081 ctactccgtt acaaagcgag gctgggtatt tcccggcctt tctgttatcc gaaatccact
     1141 gaaagcacag cggctggctg aggagataaa taataaacga ggggctgtat gcacaaagca
     1201 tcttctgttg agttaagaac gagtatcgag atggcacata gccttgctca aattggaatc
     1261 aggtttgtgc caataccagt agaaacagac gaagaagcta gctttgcact ggatggggac
     1321 gtc
//
