LOCUS       sequence-134711-       10036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains dCas9 from S. pyogenes under constitutive promoter. Suicide
            vector inserts into psba1 site of Synechocystis. Carries 
            spectinomycin resist. Recommend E. coli Copy cutter for 
            propogation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-psba1-Ppsba2-dCas9-SpR
FEATURES             Location/Qualifiers
     source          1..10036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      649..658
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1283..1304
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             join(1628..1654,1655..1657,1658..4144,4145..4147,
                     4148..5731)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5732..5761
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      5781..5852
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5868..5895
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5937..6728)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(6729..6820)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    6844..6864
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(7815..7831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7815..7831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7828..7850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7839..7855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7863..7869,7870..7887,7888..7893))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7908..7929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8046..8063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8217..8805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8297..8316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8976..9767,9768..9836))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9599..9618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9837..9941)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10009..10027
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt agaactcggt acgcgcggat
      421 cttccagaga tttatagcgt tgcagtccct ggcgggtgag tctaactccg gcaatctgct
      481 ccgctggtac cgctggaata ttgatgctaa ataaggtcgg ttccgtcaat ggcgatcgcc
      541 agaggtgttg gacaaagcaa ttggcaaagt cggcggcggg ctgaaaatcg gtggcggtaa
      601 aactgaccaa actcaaagca atgctaggaa tgccctcgat caacccttcc atggcggcgg
      661 aaactgtacc ggaatataaa acatcagtgc cgaggttagc accgtggtta actccggaaa
      721 ggacaaaatc cggataacgg ggcatcaccg catggagggc aaacttcaca cagtcagcgg
      781 gggtaccgga acaggaccaa gccttgatgc gaggatcaaa aacatcctcc acaatgcccg
      841 ctcgaatggg tcgatggagg gtcagaccat ggccggtggc ggatctttcc cggtcaggac
      901 agaccactgt gacttcatgg cctgcggcga ctagggtgtt ggccaaggta cgtacccctt
      961 gggaaaaaat gccgtcatcg ttactgacaa ggagattgag gaccggggat ggggtcatag
     1021 gacaccaaag aatcgagatt gtgcctgcca gccttaacga tatcaggcta tgtcccgctt
     1081 aaactctgat tcttaccagg taactcctgg gctccatggg gccacaacca ggcagtattt
     1141 tgttcctttg gccaatgggg cgatcgggga aaaatggctt gatctggcat ttacgagaaa
     1201 aatttttatt ttttaatgat ttattttttc ctattaaaat ctttttttta cctttggaaa
     1261 ccaactgcaa tctgagaaac cagaattcgc ggccgcttct agaggcgtgc aaggcccagt
     1321 gatcaatttc attatttttc attatttcat ctccattgtc cctgaaaatc agttgtgtcg
     1381 cccctctaca cagcccagaa ctatggtaaa ggcgcacgaa aaaccgccag gtaaactctt
     1441 ctcaaccccc aaaacgccct ctgtttaccc atggaaaaaa cgacaattac aagaaagtaa
     1501 aacttatgtc atctataagc ttcgtgtata ttaacttcct gttacaaagc tttacaaaac
     1561 tctcattaat cctttagact aagtttagtc agttccaatc tgaactaagg aattataacc
     1621 aaactagatg gataagaaat actcaatagg cttagctatc ggcacaaata gcgtcggatg
     1681 ggcggtgatc actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac
     1741 agaccgccac agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac
     1801 agcggaagcg actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg
     1861 tatttgttat ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt
     1921 tcatcgactt gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat
     1981 ttttggaaat atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct
     2041 gcgaaaaaaa ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt
     2101 agcgcatatg attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa
     2161 tagtgatgtg gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga
     2221 aaaccctatt aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa
     2281 atcaagacga ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt
     2341 tgggaatctc attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt
     2401 ggcagaagat gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt
     2461 attggcgcaa attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga
     2521 tgctatttta ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc
     2581 agcttcaatg attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt
     2641 agttcgacaa caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg
     2701 atatgcaggt tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc
     2761 aattttagaa aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt
     2821 gctgcgcaag caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga
     2881 gctgcatgct attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga
     2941 gaagattgaa aaaatcttga cttttcgcat tccttattat gttggtccat tggcgcgtgg
     3001 caatagtcgt tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt
     3061 tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt
     3121 tgataaaaat cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt
     3181 tacggtttat aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc
     3241 atttctttca ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa
     3301 agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt
     3361 tgaaatttca ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct
     3421 aaaaattatt aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga
     3481 tattgtttta acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac
     3541 atatgctcac ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg
     3601 ttggggacgt ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac
     3661 aatattagat tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca
     3721 tgatgatagt ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga
     3781 tagtttacat gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt
     3841 acagactgta aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa
     3901 tatcgttatt gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg
     3961 agagcgtatg aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga
     4021 gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa
     4081 tggaagagac atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt
     4141 cgatgccatt gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac
     4201 gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa
     4261 gatgaaaaac tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga
     4321 taatttaacg aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa
     4381 acgccaattg gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg
     4441 catgaatact aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt
     4501 aaaatctaaa ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat
     4561 taacaattac catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat
     4621 taagaaatat ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt
     4681 tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt
     4741 ttactctaat atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg
     4801 caaacgccct ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg
     4861 agattttgcc acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac
     4921 agaagtacag acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa
     4981 gcttattgct cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac
     5041 ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa
     5101 atccgttaaa gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc
     5161 gattgacttt ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact
     5221 acctaaatat agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg
     5281 agaattacaa aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt
     5341 agctagtcat tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt
     5401 tgtggagcag cataagcatt atttagatga gattattgag caaatcagtg aattttctaa
     5461 gcgtgttatt ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag
     5521 agacaaacca atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct
     5581 tggagctccc gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc
     5641 tacaaaagaa gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac
     5701 acgcattgat ttgagtcagc taggaggtga cgaacaaaaa ttgattagtg aagaagattt
     5761 gtaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     5821 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     5881 gtgggccttt ctgcgtttat atactagagc cctcggcttg aacgaattgt tagacattat
     5941 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     6001 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     6061 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     6121 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     6181 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     6241 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     6301 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     6361 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     6421 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     6481 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     6541 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     6601 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     6661 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     6721 tccctcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6781 agcggataca tatttgaatg tatttagaaa aataaacaaa tagctagctc actcggtcgc
     6841 tactactagt agcggccgct gcagtttagc ttccggtgat gcccaaatgg tggccctgaa
     6901 cgcccccgcc attgaaggct agtgccattg ccataactgc tttcggttag acttcgtttc
     6961 atttggttaa tcaagggcac tctcgcaatg gggtgccttt tatggtccaa ggttaaagtt
     7021 aagccagtaa agtttaagtc tatttctagg gtgaaatgta atgaatcaat ttagggactg
     7081 gggtttttcc actgattggt ggcaaggcaa aaaaggggaa tattgggtac ttgggcaaac
     7141 aattctctcc gttggatttg tactgttacc ggtttatact cctactaatt tacagttact
     7201 agctgaacaa aatcagtgga ttgcctgggc tggtactctg ttttttggca tgattgcggc
     7261 agttttgctg attggggggg gtctgcattt aggggaaaat ctgactccct tgccccatcc
     7321 taaaaaggat agtcaattgg tcaccactgg tatttacagc atggtccgtc atcccctcta
     7381 cggcggcgtg gtttttctgg cgatcgccta tggcttttgg caatggagtt tgacccattt
     7441 aatcggagca gtggtgttat ttatattttt taatctcaaa gctaatcggg aagaaatttg
     7501 gctccaagat aaatttaacg actatggcaa ttatcgagac caggtgaaaa agctgattcc
     7561 ttggctttat taaaggaata tgaagaaaaa tatgggaaaa aactatgtgg gatgaaagat
     7621 ttagccagtc agaatatgtt tacggtacag aaccgaatga ttttttggtc agtgtggcca
     7681 atcaaattcc ccagggaaaa attctttgtt tagcagaagg agaaggtcgt aatgcttgtt
     7741 ttttagcaag tttgggttat gaagtgaccg cggtaatcgt cgaacggcag gcgtgcaaac
     7801 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7861 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7921 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7981 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8041 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8101 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8161 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8641 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8701 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8821 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8881 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8941 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9001 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9061 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9121 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9181 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9241 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9301 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9361 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9421 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9481 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9541 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9601 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9661 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9721 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9781 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9841 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9901 tttgaatgta tttagaaaaa taaacaaatg ggggttccgc gcacatttcc ccgaaaagtg
     9961 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    10021 acgaggccct ttcgtc
//
