LOCUS       sequence-134713-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_TGIF2_1
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..300
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     52..72
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     223..242
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        320..395
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tgagggccta
       61 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
      121 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
      181 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
      241 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      301 gtctctgaaa atccccagtg ttttagagct agaaatagca agttaaaata aggctagtcc
      361 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag ctagaaatag
      421 caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag acaaatggct
      481 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      541 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      601 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      661 ccccctattg acgt
//
