LOCUS       sequence-134720-        7746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EXPRESs plasmid for human erythrocyte surface proteins encoding 
            APMAP with bio-rat CD4d3+4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, 
            Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner 
            JC, Wright GJ
  TITLE     Basigin is a receptor essential for erythrocyte invasion by 
            Plasmodium falciparum.
  JOURNAL   Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606.
  PUBMED    22080952
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-11-9- 9"; volume: "480"; issue: "7378"; pages:
            "534-7"
COMMENT     Sequence Label: bio-CD4d3+4-APMAP
FEATURES             Location/Qualifiers
     source          1..7746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1846..1901
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1900..1919)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4137..4155)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4223..4327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4328..4396,4397..5188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4546..4565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5359..5947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5848..5867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6101..6118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6235..6256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6271..6276,6277..6294,6295..6301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6309..6325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6314..6336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6333..6349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6333..6349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6620..6999
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7000..7203
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7154..7174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7200..7224
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7705..7724
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtgcc
       61 tgtgcagaca ttgtgggtcc agacctgcat catattctgg atgcacagaa aatggtgtgg
      121 aatcatcgtg acagaaacct gcctccacta gcacctctgt cgacctccat cacggcctat
      181 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
      241 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
      301 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
      361 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
      421 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
      481 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
      541 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
      601 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
      661 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttcgcggccg
      721 cccattgatc cccagcccct gagcttcaaa gagccccccc tgctgctggg agtgctgcac
      781 cccaacacca agctgcggca ggccgagaga ctgttcgaga accagctcgt gggacccgag
      841 tctatcgccc acatcggcga cgtgatgttc accggcaccg ccgacggcag agtggtcaag
      901 ctggaaaacg gcgagatcga gacaatcgcc agattcggca gcggcccctg caagaccaga
      961 gatgacgagc ccgtgtgcgg cagacctctg ggcatcagag ccggccctaa cggcaccctg
     1021 ttcgtggccg acgcctacaa gggcctgttc gaagtgaacc cctggaagcg ggaagtgaag
     1081 ctgctcctgt ccagcgagac acccatcgag ggcaagaaca tgagcttcgt gaacgacctg
     1141 acagtgaccc aggacggccg gaagatctac ttcaccgata gcagcagcaa gtggcagcgg
     1201 cgggactatc tgctgctggt catggaaggc accgacgatg gccggctgct ggaatacgac
     1261 accgtgaccc gggaggtcaa ggtgctgctg gaccagctga gattccccaa cggcgtgcag
     1321 ctgtcccctg ccgaggattt cgtgctggtc gccgagacaa ccatggccag aatccggcgg
     1381 gtgtacgtgt ctggcctgat gaagggcgga gccgacctgt ttgtggagaa catgcccggc
     1441 ttccccgaca acatcagacc tagcagctct ggcggctact gggtcggcat gagcaccatc
     1501 agacccaacc ccggcttcag catgctggac tttctgagcg agcggccctg gatcaagcgg
     1561 atgatcttca agctgttcag ccaagagaca gtgatgaagt tcgtgccccg gtacagcctg
     1621 gtgctggaac tgagcgacag cggcgccttc agaagaagcc tgcacgaccc cgatggcctg
     1681 gtggctacct acatcagcga ggtgcacgag cacgacggcc acctgtacct gggcagcttc
     1741 cggtccccct tcctgtgcag actgtctctg caggccgtgt gaggcgcgcc tgaattcttc
     1801 tagacacgtg attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta
     1861 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     1921 gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc
     1981 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     2041 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     2101 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     2161 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     2221 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     2281 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     2341 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     2401 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     2461 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     2521 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     2581 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     2641 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     2701 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     2761 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     2821 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     2881 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     2941 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     3001 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     3061 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     3121 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     3181 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     3241 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     3301 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     3361 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     3421 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     3481 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     3541 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     3601 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     3661 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     3721 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     3781 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     3841 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     3901 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     3961 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     4021 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     4081 atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct
     4141 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4201 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4321 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4381 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4441 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4501 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4561 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4621 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4681 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4741 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4801 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4861 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4921 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4981 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5041 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5101 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5161 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5221 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5281 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5461 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5821 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5881 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5941 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6001 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6061 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     6361 caagctctag ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     6421 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
     6481 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     6541 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     6601 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     6661 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     6721 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     6781 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     6841 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     6901 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     6961 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     7021 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     7081 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
     7141 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     7201 gctcgtttag tgaaccgtca gatcctcact ctcttccgca tcgctgtctg cgagggccag
     7261 ctgttgggct cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga
     7321 aacccgtcgg cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac
     7381 cggatcggaa aacctctcga gaaaggcgtc taaccagtca cagtcgcaag gtaggctgag
     7441 caccgtggcg ggcggcagcg ggtggcggtc ggggttgttt ctggcggagg tgctgctgat
     7501 gatgtaatta aagtaggcgg tcttgagacg gcggatggtc gaggtgaggt gtggcaggct
     7561 tgagatccag ctgttggggt gagtactccc tctcaaaagc gggcattact tctgcgctaa
     7621 gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgat ctggccatac
     7681 acttgagtga caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc
     7741 aagttt
//
