LOCUS       sequence-134722-        8715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EXPRESs plasmid for human erythrocyte surface proteins encoding 
            TMEM24 with bio-rat CD4d3+4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, 
            Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner 
            JC, Wright GJ
  TITLE     Basigin is a receptor essential for erythrocyte invasion by 
            Plasmodium falciparum.
  JOURNAL   Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606.
  PUBMED    22080952
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-11-9- 9"; volume: "480"; issue: "7378"; pages:
            "534-7"
COMMENT     Sequence Label: bio-CD4d3+4-TMEM24
FEATURES             Location/Qualifiers
     source          1..8715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2815..2870
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2869..2888)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5106..5124)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5192..5296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5297..5365,5366..6157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5515..5534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6328..6916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6817..6836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7070..7087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7204..7225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7240..7245,7246..7263,7264..7270)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7278..7294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7283..7305
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7302..7318
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7302..7318
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7589..7968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7969..8172
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8123..8143
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8169..8193
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     8674..8693
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtgcc
       61 tgtgcagaca ttgtgggtcc agacctgcat catattctgg atgcacagaa aatggtgtgg
      121 aatcatcgtg acagaaacct gcctccacta gcacctctgt cgacctccat cacggcctat
      181 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
      241 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
      301 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
      361 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
      421 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
      481 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
      541 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
      601 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
      661 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttcgcggccg
      721 cctcagtatg ccagaggcct gtggctggct agagccagag gcgatagagg acctggacca
      781 gccctggctg gcgaaccagc cggctctctg agagaactcg gcgtgtggcg gagcctgctg
      841 agactgagag ccacaagagc cggcgctgct gaggaacctg gcgtgagagg cctgctggca
      901 tctctgttcg ccttcaagag cttccgggag aactggcaga gagcctgggt ccgggctctg
      961 aatgagcagg cttgccggaa cggcagcagc atccagatcg ccttcgagga agtgccccag
     1021 ctgcccccta gagccagcat cagccacgtg acctgtgtgg accagagcga gcacaccatg
     1081 gtgctgagat gtcagctgtc cgccgaagaa gtgcggttcc ccgtcagcgt gacccagcag
     1141 tctcctgctg ccgtgagcat ggaaacctac cacgtgaccc tgaccctgcc ccctacccag
     1201 ctggaagtga acctggaaga gatccccggc gagggactgc tgatcagctg ggccttcacc
     1261 gacagacccg acctgagcct gaccgtgctg cccaagctgc aggccagaga gcggggcgag
     1321 gaacaggtgg aactgtccac catcgaggaa ctgatcaagg acgccatcgt gtccacccag
     1381 cccgccatga tggtcaacct gagagcctgt tctgcccctg gcggcctggt gcctagcgag
     1441 aagcccccca tgatgcctca ggcccagcct gccatcccca gacccaaccg gctgttcctg
     1501 cggcagctga gagccagcca cctgggcaac gagctggaag gcaccgagga actgtgctgc
     1561 gtggccgagc tggacaaccc catgcagcag aagtggacca agcctgccag agccggcagc
     1621 gaggtggagt ggaccgagga cctggccctg gatctgggcc ctcagagccg ggagctgacc
     1681 ctgaaggtcc tgagaagcag cagctgcggc gatacagagc tgctgggcca ggccacactg
     1741 cctgtgggca gccccagcag acctctgagc agacggcagc tgtgtcctct gacccctggc
     1801 ccaggcaaag ctctgggccc tgccgccaca atggccgtgg agctgcacta cgaggaaggc
     1861 tcccccagaa acctgggcac ccctaccagc agcaccccca gacctagcat cacccccacc
     1921 aagaagatcg agctggaccg gaccatcatg cccgacggca ccatcgtgac caccgtgaca
     1981 accgtgcaga gccggcccag aatcgacggc aagctggact ctccaagcag atcccccagc
     2041 aaggtggaag tgaccgaaaa gaccaccacc gtgctgtctg agagcagcgg ccccagcaat
     2101 accagccaca gcagcagcag ggacagccac ctgtccaacg gcctggaccc tgtggccgag
     2161 acagccatca gacagctgac cgagcctagc ggcagagtgg ccaagaaaac ccctaccaag
     2221 cggagcaccc tgatcatcag cggcgtgagc aaggtgccaa tcgcccagga tgaactcgcc
     2281 ctgagcctgg gatacgccgc cagcctggaa gccagcgtgc aggatgatgc tggcacatct
     2341 ggcggcccta gcagccctcc tagcgaccct cctgccatga gccctggccc tctggatgcc
     2401 ctgagcagcc ccacctccgt gcaggaagcc gacgagacaa ccagaagcga catcagcgag
     2461 cggcccagcg tggacgacat cgagagcgag acaggcagca caggcgccct ggaaacccgg
     2521 tccctgaagg accacaaggt gtcctttctg cggagcggca ccaagctgat cttccggcgg
     2581 aggcccagac agaaagaggc tggcctgtct cagagccacg acgacctgag caatgccacc
     2641 gccaccccct ccgtgagaaa gaaggccggg agcttcagca gacggctgat caagcggttc
     2701 agcttcaaga gcaagcccaa ggccaacggc aaccccagcc cccagctgtg aggcgcgcct
     2761 gaattcttct agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa
     2821 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     2881 catatgggag ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga
     2941 tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag
     3001 tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg
     3061 acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg
     3121 gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg
     3181 gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct
     3241 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     3301 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     3361 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     3421 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     3481 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     3541 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     3601 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     3661 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
     3721 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
     3781 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
     3841 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
     3901 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
     3961 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
     4021 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
     4081 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
     4141 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     4201 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     4261 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     4321 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     4381 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     4441 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     4501 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     4561 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     4621 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     4681 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     4741 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     4801 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4861 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4921 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     4981 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     5041 ccgggtagca tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg
     5101 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5161 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5221 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5281 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5341 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5401 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5461 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5521 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5581 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5641 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5701 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5761 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5821 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     5881 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5941 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6001 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6061 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6121 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6181 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6241 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6301 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6361 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6421 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6481 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6541 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6601 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6661 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6721 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6781 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6841 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6901 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6961 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7021 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7081 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7141 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7201 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7261 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7321 tgattacgcc aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg
     7381 cagatccggg caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc
     7441 tatacattga atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc
     7501 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
     7561 ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa
     7621 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     7681 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     7741 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     7801 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
     7861 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
     7921 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     7981 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     8041 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     8101 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     8161 ataagcagag ctcgtttagt gaaccgtcag atcctcactc tcttccgcat cgctgtctgc
     8221 gagggccagc tgttgggctc gcggttgagg acaaactctt cgcggtcttt ccagtactct
     8281 tggatcggaa acccgtcggc ctccgaacgg tactccgcca ccgagggacc tgagcgagtc
     8341 cgcatcgacc ggatcggaaa acctctcgag aaaggcgtct aaccagtcac agtcgcaagg
     8401 taggctgagc accgtggcgg gcggcagcgg gtggcggtcg gggttgtttc tggcggaggt
     8461 gctgctgatg atgtaattaa agtaggcggt cttgagacgg cggatggtcg aggtgaggtg
     8521 tggcaggctt gagatccagc tgttggggtg agtactccct ctcaaaagcg ggcattactt
     8581 ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgatc
     8641 tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact
     8701 cccaggtcca agttt
//
