LOCUS       sequence-134724-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pertussis Toxin Subunit 1 Yeast Display Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Kowalsky CA, Faber MS, Nath A, Dann HE, Kelly VW, Liu L, Shanker P, 
            Wagner EK, Maynard JA, Chan C, Whitehead TA
  TITLE     Rapid fine conformational epitope mapping using comprehensive 
            mutagenesis and deep sequencing.
  JOURNAL   J Biol Chem. 2015 Oct 30;290(44):26457-70. doi: 
            10.1074/jbc.M115.676635. Epub 2015 Aug 20.
  PUBMED    26296891
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.676635"; journalName: "J Biol Chem"; date: 
            "2015-10-30- 30"; volume: "290"; issue: "44"; pages: "26457-70"
COMMENT     Sequence Label: pETCON-PTX-S1-220
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             303..314
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             315..341
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tacttcatac attttcaatt aagatgcagt tacttcgctg tttttcaata ttttctgtta
       61 ttgcttcagt tttagcacag gaactgacaa ctatatgcga gcaaatcccc tcaccaactt
      121 tagaatcgac gccgtactct ttgtcaacga ctactatttt ggccaacggg aaggcaatgc
      181 aaggagtttt tgaatattac aaatcagtaa cgtttgtcag taattgcggt tctcacccct
      241 caacaactag caaaggcagc cccataaaca cacagtatgt ttttaaggac aatagctcga
      301 cgattgaagg tagataccca tacgacgttc cagactacgc tctgcaggct agtggtggag
      361 gaggctctgg tggaggcggt agcggaggcg gagggtcggc tagccatatg gacgatcctc
      421 ccgccaccgt ataccgctat gactcccgcc cgccggagga cgttttccag aacggattca
      481 cggcgtgggg aaacaacgac aatgtgctcg accatctgac cggacgttcc tgccaggtcg
      541 gcagcagcaa cagcgctttc gtctccacca gcagcagccg gcgctatacc gaggtctatc
      601 tcgaacatcg catgcaggaa gcggtcgagg ccgaacgcgc cggcaggggc accggccact
      661 tcatcggcta catctacgaa gtccgcgccg acaacaattt ctacggcgcc gccagctcgt
      721 acttcgaata cgtcgacact tatggcgaca atgccggccg tatcctcgcc ggcgcgctgg
      781 ccacctacca gagcgaatat ctggcacacc ggcgcattcc gcccgaaaac atccgcaggg
      841 taacgcgggt ctatcacaac ggcatcaccg gcgagaccac gaccacggag tattccaacg
      901 ctcgctacgt cagccagcag actcgcgcca atcccaaccc ctacacatcg cgaaggtccg
      961 tagcgtcgat cgtcggcaca t
//
