LOCUS       sequence-134727-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tumor Necrosis Factor Alpha Yeast Display Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Kowalsky CA, Faber MS, Nath A, Dann HE, Kelly VW, Liu L, Shanker P, 
            Wagner EK, Maynard JA, Chan C, Whitehead TA
  TITLE     Rapid fine conformational epitope mapping using comprehensive 
            mutagenesis and deep sequencing.
  JOURNAL   J Biol Chem. 2015 Oct 30;290(44):26457-70. doi: 
            10.1074/jbc.M115.676635. Epub 2015 Aug 20.
  PUBMED    26296891
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.676635"; journalName: "J Biol Chem"; date: 
            "2015-10-30- 30"; volume: "290"; issue: "44"; pages: "26457-70"
COMMENT     Sequence Label: pETCON-TNF
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(293..322)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 attcatcttt ttttagctac taataggata aattatagga atttataaca aattgaaata
       61 tggcaggcag caaaattaaa attgtcttag ttttttagtg tatagaagtg aatagctata
      121 taaagtatgt gtaaagttgg taacggaacg aaaaatagaa aaggatatta catgggaaaa
      181 catgttgttt acggagaaat gaaaagtata ttgtattttg tacgagctaa aagtacagtg
      241 ggaacaaagt cgattttgtt acatctacac tgttgttatc agatctctat tacaagtcct
      301 cttcagaaat aagcttttgt tcggatccgc ccccctcgag cagggcgatg atgccgaagt
      361 acacctggcc gctctcggcg aagtccaggt agtcgggcct gttgatctcg gcgctcagcc
      421 tgtcgccctt ctccagctgg aacacgccgc ccaggtagat gggctcgtac cagggcttgg
      481 cctcggcgcc ctcgggggtc tccctctggc aggggctctt gatggcgctc agcaggttca
      541 ccttggtctg gtagctcacg gcgatcctgc tgatggtgtg ggtcagcagc acgtgggtgc
      601 tggggcagcc ctggcccttg aacagcacct ggctgtagat caggtacagg ccctcgctgg
      661 gcaccaccag ctggttgtcc ctcagctcca cgccgttggc cagcagggcg ttggccctcc
      721 tgttcagcca ctgcagctgg ccctcggcct gggggttggc caccacgtgg gccacgggct
      781 tgtcgctggg ggtcctgctg ctgctcctca cggcctgggc cagggggctg atca
//
