LOCUS       sequence-134734-         100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 100)
  AUTHORS   Gao S, Tong Y, Wen Z, Zhu L, Ge M, Chen D, Jiang Y, Yang S
  TITLE     Multiplex gene editing of the Yarrowia lipolytica genome using the 
            CRISPR-Cas9 system.
  JOURNAL   J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi: 
            10.1007/s10295-016-1789-8. Epub 2016 Jun 27.
  PUBMED    27349768
REFERENCE   2  (bases 1 to 100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10295-016-1789-8"; journalName: "J Ind Microbiol 
            Biotechnol"; date: "2016-08"; volume: "43"; issue: "8"; pages: 
            "1085-93"
COMMENT     Sequence Label: pCAS1yl
FEATURES             Location/Qualifiers
     source          1..100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        21..96
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 actggatcag gacgccatcg gttttagagc tagaaatagc aagttaaaat aaggctagtc
       61 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
//
