LOCUS       sequence-134740-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tumor Necrosis Factor Alpha Yeast Display Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Kowalsky CA, Faber MS, Nath A, Dann HE, Kelly VW, Liu L, Shanker P, 
            Wagner EK, Maynard JA, Chan C, Whitehead TA
  TITLE     Rapid fine conformational epitope mapping using comprehensive 
            mutagenesis and deep sequencing.
  JOURNAL   J Biol Chem. 2015 Oct 30;290(44):26457-70. doi: 
            10.1074/jbc.M115.676635. Epub 2015 Aug 20.
  PUBMED    26296891
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.676635"; journalName: "J Biol Chem"; date: 
            "2015-10-30- 30"; volume: "290"; issue: "44"; pages: "26457-70"
COMMENT     Sequence Label: pETCON-TNF
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             303..314
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             315..341
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tacttcatac attttcaatt aagatgcagt tacttcgctg tttttcaata ttttctgtta
       61 ttgcttcagt tttagcacag gaactgacaa ctatatgcga gcaaatcccc tcaccaactt
      121 tagaatcgac gccgtactct ttgtcaacga ctactatttt ggccaacggg aaggcaatgc
      181 aaggagtttt tgaatattac aaatcagtaa cgtttgtcag taattgcggt tctcacccct
      241 caacaactag caaaggcagc cccataaaca cacagtatgt ttttaaggac aatagctcga
      301 cgattgaagg tagataccca tacgacgttc cagactacgc tctgcaggct agtggtggag
      361 gaggctctgg tggaggcggt agcggaggcg gagggtcggc tagccatatg ggcccccaga
      421 gggaggagtt ccccagggac ctgagcctga tcagccccct ggcccaggcc gtgaggagca
      481 gcagcaggac ccccagcgac aagcccgtgg cccacgtggt ggccaacccc caggccgagg
      541 gccagctgca gtggctgaac aggagggcca acgccctgct ggccaacggc gtggagctga
      601 gggacaacca gctggtggtg cccagcgagg gcctgtacct gatctacagc caggtgctgt
      661 tcaagggcca gggctgcccc agcacccacg tgctgctgac ccacaccatc agcaggatcg
      721 ccgtgagcta ccagaccaag gtgaacctgc tgagcgccat caagagcccc tgccagaggg
      781 agacccccga gggcgccgag gccaagccct ggtacgagcc catctacctg ggcggcgtgt
      841 tccagctgga gaagggcgac aggctgagcg ccgagatcaa caggcccgac tacctggact
//
