LOCUS       sequence-134742-          61 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of cas9 and inducible expression of lambda 
            RED and sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 61)
  AUTHORS   Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S
  TITLE     A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome 
            editing in Escherichia coli.
  JOURNAL   Acta Biochim Biophys Sin (Shanghai). 2021 Apr 15;53(5):620-627. doi:
            10.1093/abbs/gmab036.
  PUBMED    33764372
REFERENCE   2  (bases 1 to 61)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Acta Biochim Biophys Sin (Shanghai)"; date: "2021-04-15- 15"; 
            volume: "53"; issue: "5"; pages: "620-627"
COMMENT     Sequence Label: pEcCas
FEATURES             Location/Qualifiers
     source          1..61
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
       61 g
//
