LOCUS       sequence-134744-        1210 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1210)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AKAP13
FEATURES             Location/Qualifiers
     source          1..1210
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt ccaaacagct ggaagcagag tcttggagtc ggataataga cagcaagttt
      121 ctaaaacagc aaaagaaaga tgtggtcaaa cggcaagaag taatatatga gttgatgcag
      181 acagagtttc atcatgtccg cactctcaag atcatgagtg gtgtgtacag ccaggggatg
      241 atggcggatc tgctttttga gcagcagatg gtagaaaagc tgttcccctg tttggatgag
      301 ctgatcagta tccatagcca attcttccag aggattctgg agcggaagaa ggagtctctg
      361 gtggataaaa gtgaaaagaa ctttctcatc aagaggatag gggatgtgct tgtaaatcag
      421 ttttcaggtg agaatgcaga acgtttaaag aagacatatg gcaagttttg tgggcaacat
      481 aaccagtctg taaactactt caaagacctt tatgccaagg ataagcgttt tcaagccttt
      541 gtaaagaaga agatgagcag ttcagttgtt agaaggcttg gaattccaga gtgcatattg
      601 cttgtaactc agcggattac caagtaccca gttttattcc aaagaatatt gcagtgtacc
      661 aaagacaatg aagtggagca ggaagatcta gcacagtcct tgagcctggt gaaggatgtg
      721 attggagctg tagacagcaa agtggcaagt tatgaaaaga aagtgcgtct caatgagatt
      781 tatacaaaga cagatagcaa gtcaatcatg aggatgaaga gtggtcagat gtttgccaag
      841 gaagatttga aacggaagaa gcttgtacgt gatgggagtg tgtttctgaa gaatgcagca
      901 ggaaggttga aagaggttca agcagttctt ctcactgaca ttttagtttt ccttcaagaa
      961 aaagaccaga agtacatctt tgcatcattg gaccagaagt caacagtgat ctctttaaag
     1021 aagctgattg tgagagaagt ggcacatgag gagaaaggtt tattcctgat cagcatgggg
     1081 atgacagatc cagagatggt agaagtccat gccagctcca aagaggaacg aaacagctgg
     1141 attcagatca ttcaggacac aatcaacacc ctgaacagag attgacagta aaggtggata
     1201 cggatccgaa
//
