LOCUS       sequence-134747-        1312 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1312)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SEC14L4
FEATURES             Location/Qualifiers
     source          1..1312
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt cgtcccgtgt gggtgacctg tcgccgcaac aacaagaagc cctggcccgc
      121 ttccgtgaaa acctgcaaga cctgctgccg attctgccga acgcggatga ctattttctg
      181 ctgcgttggc tgcgtgcccg caatttcgat ctgcagaaaa gtgaagacat gctgcgtcgt
      241 cacatggaat ttcgcaaaca gcaggatctg gataacattg tcacctggca gccgccggaa
      301 gtgatccaac tgtatgattc aggcggtctg tgcggctatg actacgaagg ttgtccggtg
      361 tacttcaata ttatcggcag cctggacccg aaaggtctgc tgctgagtgc ttccaagcag
      421 gacatgattc gtaaacgcat caaggtgtgc gaactgctgc tgcatgaatg tgaactgcag
      481 acccaaaaac tgggccgtaa gattgaaatg gcgctgatgg tttttgatat ggaaggtctg
      541 agtctgaaac acctgtggaa gccggcagtg gaagtttatc agcaattttt ctccatcctg
      601 gaagctaact acccggaaac gctgaaaaat ctgattgtta tccgcgcgcc gaagctgttt
      661 ccggtggcct tcaacctggt taaatcattc atgtcggaag aaacccgtcg caaaattgtg
      721 atcctgggcg ataattggaa acaggaactg acgaagttta ttagcccgga ccaactgccg
      781 gttgaatttg gcggcaccat gaccgatccg gacggtaacc cgaaatgcct gaccaagatc
      841 aattatggcg gtgaagtgcc gaaaagctat tacctgtgtg aacaggttcg tctgcaatac
      901 gaacacacgc gttctgttgg ccgcggtagc tctctgcagg tcgaaaacga aattctgttt
      961 ccgggttgcg ttctgcgttg gcagttcgca agcgatggcg gtgacatcgg ctttggtgtc
     1021 ttcctgaaaa ccaagatggg tgaacagcaa tcagcgcgtg aaatgacgga agtcctgccg
     1081 tcgcagcgct ataacgccca catggtgccg gaagatggct ctctgacctg tctgcaggca
     1141 ggtgtctatg tgctgcgttt tgataatacc tacagtcgca tgcacgcgaa aaagctgtcc
     1201 tacacggttg aagtcctgct gccggataaa gcaagcgaag aaaccctgca gtctctgaaa
     1261 gctatgcgtc cgagcccgac gcaatgacag taaaggtgga tacggatccg aa
//
