LOCUS       sequence-134749-        1234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1234)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAPK3
FEATURES             Location/Qualifiers
     source          1..1234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg cggcggcggc ggctcagggg ggcgggggcg gggagccccg tagaaccgag
      121 ggggtcggcc cgggggtccc gggggaggtg gagatggtga aggggcagcc gttcgacgtg
      181 ggcccgcgct acacgcagtt gcagtacatc ggcgagggcg cgtacggcat ggtcagctcg
      241 gcctatgacc acgtgcgcaa gactcgcgtg gccatcaaga agatcagccc cttcgaacat
      301 cagacctact gccagcgcac gctccgggag atccagatcc tgctgcgctt ccgccatgag
      361 aatgtcatcg gcatccgaga cattctgcgg gcgtccaccc tggaagccat gagagatgtc
      421 tacattgtgc aggacctgat ggagactgac ctgtacaagt tgctgaaaag ccagcagctg
      481 agcaatgacc atatctgcta cttcctctac cagatcctgc ggggcctcaa gtacatccac
      541 tccgccaacg tgctccaccg agatctaaag ccctccaacc tgctcatcaa caccacctgc
      601 gaccttaaga tttgtgattt cggcctggcc cggattgccg atcctgagca tgaccacacc
      661 ggcttcctga cggagtatgt ggctacgcgc tggtaccggg ccccagagat catgctgaac
      721 tccaagggct ataccaagtc catcgacatc tggtctgtgg gctgcattct ggctgagatg
      781 ctctctaacc ggcccatctt ccctggcaag cactacctgg atcagctcaa ccacattctg
      841 ggcatcctgg gctccccatc ccaggaggac ctgaattgta tcatcaacat gaaggcccga
      901 aactacctac agtctctgcc ctccaagacc aaggtggctt gggccaagct tttccccaag
      961 tcagactcca aagcccttga cctgctggac cggatgttaa cctttaaccc caataaacgg
     1021 atcacagtgg aggaagcgct ggctcacccc tacctggagc agtactatga cccgacggat
     1081 gagccagtgg ccgaggagcc cttcaccttc gccatggagc tggatgacct acctaaggag
     1141 cggctgaagg agctcatctt ccaggagaca gcacgcttcc agcccggagt gctggaggcc
     1201 ccctagtgac agtaaaggtg gatacggatc cgaa
//
