LOCUS       sequence-134750-        1183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1183)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAPK8
FEATURES             Location/Qualifiers
     source          1..1183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga gcagaagcaa gcgtgacaac aatttttata gtgtagagat tggagattct
      121 acattcacag tcctgaaacg atatcagaat ttaaaaccta taggctcagg agctcaagga
      181 atagtatgcg cagcttatga tgccattctt gaaagaaatg ttgcaatcaa gaagctaagc
      241 cgaccatttc agaatcagac tcatgccaag cgggcctaca gagagctagt tcttatgaaa
      301 tgtgttaatc acaaaaatat aattggcctt ttgaatgttt tcacaccaca gaaatcccta
      361 gaagaatttc aagatgttta catagtcatg gagctcatgg atgcaaatct ttgccaagtg
      421 attcagatgg agctagatca tgaaagaatg tcctaccttc tctatcagat gctgtgtgga
      481 atcaagcacc ttcattctgc tggaattatt catcgggact taaagcccag taatatagta
      541 gtaaaatctg attgcacttt gaagattctt gacttcggtc tggccaggac tgcaggaacg
      601 agttttatga tgacgcctta tgtagtgact cgctactaca gagcacccga ggtcatcctt
      661 ggcatgggct acaaggaaaa cgtggattta tggtctgtgg ggtgcattat gggagaaatg
      721 gtttgccaca aaatcctctt tccaggaagg gactatattg atcagtggaa taaagttatt
      781 gaacagcttg gaacaccatg tcctgaattc atgaagaaac tgcaaccaac agtaaggact
      841 tacgttgaaa acagacctaa atatgctgga tatagctttg agaaactctt ccctgatgtc
      901 cttttcccag ctgactcaga acacaacaaa cttaaagcca gtcaggcaag ggatttgtta
      961 tccaaaatgc tggtaataga tgcatctaaa aggatctctg tagatgaagc tctccaacac
     1021 ccgtacatca atgtctggta tgatccttct gaagcagaag ctccaccacc aaagatccct
     1081 gacaagcagt tagatgaaag ggaacacaca atagaagagt ggaaagaatt gatatataag
     1141 gaagttatgg acttgtgaca gtaaaggtgg atacggatcc gaa
//
