LOCUS       sequence-134752-         493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 493)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBS
FEATURES             Location/Qualifiers
     source          1..493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga agccctggtg gtggcacctc cgtgttcagg agctgggcct gtcagccccg
      121 ctgaccgtgc tcccgaccat cacctgtggg cacaccatcg agatcctccg ggagaagggc
      181 ttcgaccagg cgcccgtggt ggatgaggcg ggggtaatcc tgggaatggt gacgcttggg
      241 aacatgctct cgtccctgct tgccgggaag gtgcagccgt cagaccaagt tggcaaagtc
      301 atctacaagc agttcaaaca gatccgcctc acggacacgc tgggcaggct ctcgcacatc
      361 ctggagatgg accacttcgc cctggtggtg cacgagcagc agcggcagat ggtgttcggg
      421 gtggtcaccg ccattgactt gctgaacttc gtggccgccc aggagtgaca gtaaaggtgg
      481 atacggatcc gaa
//
