LOCUS       sequence-134754-        1294 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1294)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SEC14L3
FEATURES             Location/Qualifiers
     source          1..1294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga gcggccgagt tggagacctg agccccaaac aggcagagac cctggccaag
      121 ttccgagaaa acgtccagga tgtgcttcct gccctgccca accctgatga ttatttcctt
      181 ctacgctggc tccgagctcg gaattttgac ttgcagaagt cggaggcttt gctccgcaag
      241 tacatggagt tccggaagac catggatatt gaccatatcc ttgattggca gcccccagag
      301 gtgatccaga agtacatgcc tgggggcctg tgtggctatg accgtgatgg ctgccccgtg
      361 tggtatgaca tcattgggcc acttgatccc aaggggttgc tcttctcagt caccaagcag
      421 gacctgctca agaccaagat gagggactgt gagcgcatcc tgcatgagtg tgacctgcag
      481 acagagaggc tagggaagaa gattgagacc atcgtgatga tatttgactg tgagggcctg
      541 ggactgaaac acttctggaa acctctggta gaagtgtacc aggagttctt tggcctcctt
      601 gaagagaatt acccagagac cctgaagttc atgctcatcg tgaaagctac caaactgttc
      661 cctgtgggct acaacctcat gaagccattc ctgagtgagg acactcgcag gaaaattatt
      721 gtgttgggaa ataactggaa ggaaggtttg ctgaaactca tcagtcctga ggaactgcct
      781 gcccagtttg ggggcaccct gactgaccca gatgggaacc ccaaatgttt aaccaagatt
      841 aactatggcg gggagatccc caagtccatg tacgtgcggg accaggtgaa gactcagtac
      901 gagcactcgg tgcagatcaa ccgcggctca tcacaccaag tggaatacga gatcctattt
      961 ccaggctgcg ttctcaggtg gcagttctca tctgacggtg cggacatcgg cttcggagtt
     1021 ttcctgaaga ccaagatggg ggagcgacag cgggcagggg agatgacaga ggttctaccc
     1081 agccagcgct ataacgccca catggtgccc gaggatggga acctcacctg ctcagaggcc
     1141 ggcgtctatg tcctacgctt cgacaacacc tatagctttg tccacgccaa gaaggtcagc
     1201 ttcacagtgg aggtcctgct ccctgacgag ggcatgcaga aatatgataa ggagctcacc
     1261 cctgtctgac agtaaaggtg gatacggatc cgaa
//
