LOCUS       sequence-134757-        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TPH2
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc tagaggatgt gccctggttc cctcggaaga tctctgagtt agacaaatgc
      121 tctcacagag ttctcatgta tggttctgag cttgatgctg accacccagg atttaaggac
      181 aatgtctatc gacagagaag aaagtatttt gtggatgtgg ccatgggtta taaatatggt
      241 cagcccattc ccagggtgga gtatactgaa gaagaaacta aaacttgggg tgttgtattc
      301 cgggagctct ccaaactcta tcccactcat gcttgccgag agtatttgaa aaacttccct
      361 ctgctgacta aatactgtgg ctacagagag gacaatgtgc ctcaactcga agatgtctcc
      421 atgtttctga aagaaaggtc tggcttcacg gtgaggccgg tggctggata cctgagccca
      481 cgagactttc tggcaggact ggcctacaga gtgttccact gtacccagta catccggcat
      541 ggctcagatc ccctctacac cccggaacca gacacatgcc atgaactctt gggacatgtt
      601 ccactacttg cggatcctaa gtttgctcag ttttcacaag aaataggtct ggcgtctctg
      661 ggagcatcag atgaagatgt tcagaaacta gccacgtgct atttcttcac aatcgagttt
      721 ggcctttgca agcaagaagg gcaactgcgg gcttatggag caggactcct ttcctccatt
      781 ggagaattaa agcacgccct ttctgacaag gcatgtgtga aagcctttga cccaaagaca
      841 acttgcttac aggaatgcct tatcaccacc ttccaggaag cctactttgt ttcagaaagt
      901 tttgaagaag ccaaagaaaa gatgagggac tttgcaaagt caattacccg tcccttctca
      961 gtatacttca atccctacac acagagtatt gaaattctga aagacaccag aagtattgaa
     1021 aatgtggtgc aggaccttcg cagcgacttg aatacagtgt gtgatgcttt aaacaaaatg
     1081 aaccaatatc tggggatttg acagtaaagg tggatacgga tccgaa
//
