LOCUS       sequence-134758-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAK1
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1024..1044
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1045..1062
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgacctcgg gcctgggcag tggctacatc ggaagagtct tcggcatcgg gcgacagcag
       61 gtcacagtgg acgaggtgtt ggcggaaggt ggatttgcta ttgtatttct ggtgaggaca
      121 agcaatggga tgaaatgtgc cttgaaacgc atgtttgtca acaatgagca tgatctccag
      181 gtgtgcaaga gagaaatcca gataatgagg gatctttcag ggcacaagaa tattgtgggt
      241 tacattgatt ctagtatcaa caacgtgagt agcggtgatg tatgggaagt gctcattctg
      301 atggactttt gtagaggtgg ccaggtggta aacctgatga accagcgcct gcaaacaggc
      361 tttacagaga atgaagtgct ccagatattt tgtgatacct gtgaagctgt tgcccgcctg
      421 catcagtgca aaactcctat tatccaccgg gacctgaagg ttgaaaacat cctcttgcat
      481 gaccgaggcc actatgtcct gtgtgacttt ggaagcgcca ccaacaaatt ccagaatcca
      541 caaactgagg gagtcaatgc agtagaagat gagattaaga aatacacaac gctgtcctat
      601 cgagcaccag aaatggtcaa cctgtacagt ggcaaaatca tcactacgaa ggcagacatt
      661 tgggctcttg gatgtttgtt gtataaatta tgctacttca ctttgccatt tggggaaagt
      721 caggtggcaa tttgtgatgg aaacttcaca attcctgata attctcgata ttctcaagac
      781 atgcactgcc taattaggta tatgttggaa ccagaccctg acaaaaggcc ggatatttac
      841 caggtgtcct acttctcatt taagctactc aagaaagagt gcccaattcc aaatgtacag
      901 aactctccca ttcctgcaaa gcttcctgaa ccagtgaaag ccagtgaggc agctgcaaaa
      961 aagacccagc caaaggccag actgacagat cccattccca ccacagagac ttcaattgca
     1021 gcagagaacc tctacttcca atcgcaccat catcaccacc attgagcaag aggatccg
//
