LOCUS       sequence-134759-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ASB11
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ctgatcgatc cccacttcat gaagctgcag ctcaggggcg cttactggcc
      121 cttaaaactt taattgcaca aggtgtcaat gtgaaccttg tgacaattaa ccgggtgtct
      181 tctctccacg aggcatgcct tggaggtcac gtggcctgtg ccaaagcctt attggaaaat
      241 ggtgcacacg tcaatggagt gacagttcac ggagccacac ccctcttcaa tgcttgctgc
      301 agcggcagtg ctgcatgtgt caatgtgctg ctggagttcg gagccaaggc ccagttggag
      361 gtgcacctgg cctcgcccat ccatgaggca gtgaagagag gtcacagaga gtgcatggag
      421 atcctgctgg caaataatgt taacattgac catgaggtgc ctcagctcgg aactccccta
      481 tatgtggcct gcacctacca gagggtagac tgtgtgaaga aacttctaga attaggagcc
      541 agtgtcgacc atggccagtg gctggacacc ccactccatg ctgcagcgag gcagtccaat
      601 gtggaggtca tccacctgct aaccgactat ggagctaacc tgaagcgtag aaatgctcag
      661 ggcaaaagtg cgcttgatct ggcggctcca aaaagcagcg tggagcaggc actcttgctc
      721 cgtgaaggcc cacctgctct ttgacagtaa aggtggata
//
