LOCUS       sequence-134762-        1174 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1174)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAPK1
FEATURES             Location/Qualifiers
     source          1..1174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1111..1146
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg cggcggcggc ggcggcgggc gcgggcccgg agatggtccg cgggcaggtg
      121 ttcgacgtgg ggccgcgcta caccaacctc tcgtacatcg gcgagggcgc ctacggcatg
      181 gtgtgctctg cttatgataa tgtcaacaaa gttcgagtag ctatcaagaa aatcagcccc
      241 tttgagcacc agacctactg ccagagaacc ctgagggaga taaaaatctt actgcgcttc
      301 agacatgaga acatcattgg aatcaatgac attattcgag caccaaccat cgagcaaatg
      361 aaagatgtat atatagtaca ggacctcatg gaaacagatc tttacaagct cttgaagaca
      421 caacacctca gcaatgacca tatctgctat tttctctacc agatcctcag agggttaaaa
      481 tatatccatt cagctaacgt tctgcaccgt gacctcaagc cttccaacct gctgctcaac
      541 accacctgtg atctcaagat ctgtgacttt ggcctggccc gtgttgcaga tccagaccat
      601 gatcacacag ggttcctgac agaatatgtg gccacacgtt ggtacagggc tccagaaatt
      661 atgttgaatt ccaagggcta caccaagtcc attgatattt ggtctgtagg ctgcattctg
      721 gcagaaatgc tttctaacag gcccatcttt ccagggaagc attatcttga ccagctgaac
      781 cacattttgg gtattcttgg atccccatca caagaagacc tgaattgtat aataaattta
      841 aaagctagga actatttgct ttctcttcca cacaaaaata aggtgccatg gaacaggctg
      901 ttcccaaatg ctgactccaa agctctggac ttattggaca aaatgttgac attcaaccca
      961 cacaagagga ttgaagtaga acaggctctg gcccacccat atctggagca gtattacgac
     1021 ccgagtgacg agcccatcgc cgaagcacca ttcaagttcg acatggaatt ggatgacttg
     1081 cctaaggaaa agctcaaaga actaattttt gaagagactg ctagattcca gccaggatac
     1141 agatcttgac agtaaaggtg gatacggatc cgaa
//
