LOCUS       sequence-134768-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GYG2
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             802..822
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             823..840
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             841..864
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgactgatc aggcttttgt cacactagcc accaatgaca tctactgcca gggcgccctg
       61 gtcctggggc agtcactgag gagacacagg ctgacgagga agctggtggt gttgatcact
      121 cctcaggtgt ccgatctgct caggcgcatc ctctcgaagg tgttcgatga agtcattgaa
      181 gtgaatctaa tcgatagtgc cgactacatc cacctggcct ttctgaagag acctgagctc
      241 gggctcaccc tcaccaagct tcactgttgg actctcactc actacagcaa gtgtgtcttc
      301 ctggatgcag acactctggt gctgtccaat gtcgatgagc tgtttgacag gggagagttt
      361 tctgcggccc cggaccccgg atggccggat tgcttcaata gcggggtgtt tgtcttccag
      421 ccttctctcc acacgcataa actcctgcta cagcacgcca tggaacacgg cagctttgac
      481 ggggcagacc aaggcttact gaatagtttc ttcaggaact ggtcgaccac agacatccac
      541 aagcacctgc cgttcatcta taacttgagt agtaacacga tgtacactta cagccctgcc
      601 ttcaagcaat tcggttccag tgcaaaggtc gtccactttt tggggtccat gaaaccttgg
      661 aactacaagt acaatccaca gagtggctcg gtgttggagc aaggctcagt gtccagcagc
      721 cagcaccagg cggcattcct tcatctctgg tggacggtct accagaacaa cgtgctgccc
      781 ctttataaaa gcgtccaagc agagaacctc tacttccaat cgcaccatca tcaccaccat
      841 gattacaagg atgacgacga taagtga
//
