LOCUS       sequence-134769-         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SHC1
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gctggctgca tcccaacgac aaagtcatgg gacccggggt ttcctacttg
      121 gttcggtaca tgggttgtgt ggaggtcctc cagtcaatgc gtgccctgga cttcaacacc
      181 cggactcagg tcaccaggga ggccatcagt ctggtgtgtg aggctgtgcc gggtgctaag
      241 ggggcgacaa ggaggagaaa gccctgtagc cgcccgctca gctctatcct ggggaggagt
      301 aacctgaaat ttgctggaat gccaatcact ctcaccgtct ccaccagcag cctcaacctc
      361 atggccgcag actgcaaaca gatcatcgcc aaccaccaca tgcaatctat ctcatttgca
      421 tccggcgggg atccggacac agccgagtat gtcgcctatg ttgccaaaga ccctgtgaat
      481 cagagagcct gccacattct ggagtgtccc gaagggcttg cccaggatgt catcagcacc
      541 attggccagg ccttcgagtt gcgctgacag taaaggtgga tacggatccg aa
//
