LOCUS       sequence-134771-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCNK2
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             850..870
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             871..900
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             901..924
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttaagaagga gatatactat gcccacagtg cttgcttccc gggtggagag tgacacgacc
       61 attaatgtta tgaaatggaa gacggtctcc acgatattcc tggtggttgt cctctatctg
      121 atcatcggag ccaccgtgtt caaagcattg gagcagcctc atgagatttc acagaggacc
      181 accattgtga tccagaagca aacattcata tcccaacatt cctgtgtcaa ttcgacggag
      241 ctggatgaac tcattcagca aatagtggca gcaataaatg cagggattat accgttagga
      301 aacacctcca atcaaatcag tcactgggat ttgggaagtt ccttcttctt tgctggcact
      361 gttattacaa ccataggatt tggaaacatc tcaccacgca cagaaggcgg caaaatattc
      421 tgtatcatct atgccttact gggaattccc ctctttggtt ttctcttggc tggagttgga
      481 gatcagctag gcaccatatt tggaaaagga attgccaaag tggaagatac gtttattaag
      541 tggaatgtta gtcagaccaa gattcgcatc atctcaacaa tcatatttat actatttggc
      601 tgtgtactct ttgtggctct gcctgcgatc atattcaaac acatagaagg ctggagtgcc
      661 ctggacgcca tttattttgt ggttatcact ctaacaacta ttggatttgg tgactacgtt
      721 gcaggtggat ccgatattga atatctggac ttctataagc ctgtcgtgtg gttctggatc
      781 cttgtagggc ttgcttactt tgctgctgtc ctgagcatga ttggagattg gctccgagtg
      841 atatctgcag agaacctcta cttccaatcg caccatcatc accatcacca tcaccaccat
      901 gattacaagg atgacgacga taagtgagga tcc
//
