LOCUS       sequence-134774-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPHK1
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgggccacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggagccgcg ggtcgaggtt atggatccag cgggcggccc ccggggcgtg
      121 ctcccgcggc cctgccgcgt gctggtgctg ctgaacccgc gcggtggcaa gggcaaggcc
      181 ttgcagctct tccggagtca cgtgcagccc cttttggctg aggctgaaat ctccttcacg
      241 ctgatgctca ctgagcggcg gaaccacgcg cgggagctgg tgcggtcgga ggagctgggc
      301 cgctgggacg ctctggtggt catgtctgga gacgggctga tgcacgaggt ggtgaacggg
      361 ctcatggagc ggcctgactg ggagaccgcc atccagaagc ccctgtgtag cctcccagca
      421 ggctctggca acgcgctggc agcttccttg aaccattatg ctggctatga gcaggtcacc
      481 aatgaagacc tcctgaccaa ctgcacgcta ttgctgtgcc gccggctgct gtcacccatg
      541 aacctgctgt ctctgcacac ggcttcgggg ctgcgcctct tctctgtgct cagcctggcc
      601 tggggcttca ttgctgatgt ggacctagag agtgagaagt atcggcgtct gggggagatg
      661 cgcttcactc tgggcacctt cctgcgtctg gcagccctgc gcacctaccg cggccgactg
      721 gcctacctcc ctgtaggaag agtgggttcc aagacacctg cctcccccgt tgtggtccag
      781 cagggcccgg tagatgcaca ccttgtgcca ctggaggagc cagtgccctc tcactggaca
      841 gtggtgcccg acgaggactt tgtgctagtc ctggcactgc tgcactcgca cctgggcagt
      901 gagatgtttg ctgcacccat gggccgctgt gcagctggcg tcatgcatct gttctacgtg
      961 cgggcgggag tgtctcgtgc catgctgctg cgcctcttcc tggccatgga gaagggcagg
     1021 catatggagt atgaatgccc ctacttggta tacgtgcccg tggtcgcctt ccgtttggag
     1081 cccaaggatg ggaaaggtgt gtttgcagtg gatggggaat tgatggttag cgaggccgtg
     1141 cagggccagg tgcacccaaa ctacttctgg atggtcagct gacagtaaag gtggatacgg
     1201 atccgaattc gagctccgtc gacaagctt
//
