LOCUS       sequence-134776-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human ZNF146.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_ZNF146
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..74)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(90..113)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(117..140)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(144..167)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcatgcgaa acgatccagg tccagggttc tcctccacgt ctccagcctg cttcagcagg
       61 ctgaagttag tagctccgct tcctgaaacc ttatcgtcgt catccttgta atctccctta
      121 tcgtcgtcat ccttgtaatc gcccttatcg tcgtcatcct tgtaatctcc agaaccacct
      181 ccaccggaac ctcctccgct cccgtgagta tgaattttct ggtgtctaat gtggtgtgac
      241 ttctggctga aagctttccc acattcactg cactgataag gcttcttacc tgtgtgtatt
      301 ctcaaatgca gagcaagggt tgagaactga gagaaagctt tcccacattc attacaacca
      361 tagggcttct cccctgtatg gcttctcaca tgcacagtga gagatgagct ctgagagaag
      421 gcttttccac aaacattgca ttcgtaaggt ttatcacctg aatgaatcct cacatgtaca
      481 ataagtgatg ttcgctgaga gaaggctttt ccacattcgt tacattcata gggtttctct
      541 ccagtgtgaa tgttctgatg ttttatgagg tacttcttct ggccaaaggc tgttccacat
      601 tcactacatt taaaaggctt ctctccaata tggattttct catgctcagt aaggttggat
      661 ttgccactga aggtttttcc acactcctta catacaaagg gcttctctcc tgtgtgagtt
      721 ctctggtgtc tgatgaggtt tgacttctga atgaaagctt tcccgcaatc tttacactca
      781 aaaggttttt ctccagtgtg a
//
