LOCUS       sequence-134779-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human DNMT3B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_DNMT3B
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..74)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(90..113)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(117..140)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(144..167)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcatgcgaa acgatccagg tccagggttc tcctccacgt ctccagcctg cttcagcagg
       61 ctgaagttag tagctccgct tcctgaaacc ttatcgtcgt catccttgta atctccctta
      121 tcgtcgtcat ccttgtaatc gcccttatcg tcgtcatcct tgtaatctcc agaaccacct
      181 ccaccggaac ctcctccgct cccttcacat gcaaagtagt ccttcagagg ggcgaagagg
      241 tgtcggatga caggcacgct ccaggacctt cccagcagct tctggcgggc accacggccc
      301 atgttggaca cgtctgtgta gtgcacagga aagccaaaga tcctgggggt accggagtga
      361 aaagacagca agtcagaagt gcctccagga tgttgacagc ctcaaccagc caggtcccac
      421 tctttctgcc ctcgccatcc ctcagtccca tcagtaaggt ccccaaggac ctaaattcag
      481 agcccttaat tcagaacact tgcttacaaa ttgctcacaa atgaaagtcc cccgttttgt
      541 gcaggcctag caccatactc ttggcataga gttggtgctc agaggacctt cctagaataa
      601 atgcatgcat actaaatcag ccagccagca atgtatccat tttcaaaaca ctatgatggg
      661 agaagttaag caatttcctt aataaggaac aaggtcactc aaaaacagcc tagattttca
      721 gtcatcacag gctcaggcaa ccagagtttt ccttccctct tcaaacccag ctgccggatg
      781 a
//
