LOCUS       sequence-134781-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human TGIF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_TGIF2
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..74)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(90..113)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(117..140)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(144..167)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcatgcgaa acgatccagg tccagggttc tcctccacgt ctccagcctg cttcagcagg
       61 ctgaagttag tagctccgct tcctgaaacc ttatcgtcgt catccttgta atctccctta
      121 tcgtcgtcat ccttgtaatc gcccttatcg tcgtcatcct tgtaatctcc agaaccacct
      181 ccaccggaac ctcctccgct cccttgggga ttttcagaga ctaaagggat gggagtgtgc
      241 agtaatggga gtgatgggtc ctgctgcttc tgaagctcca tctcagcagc cctctgtagc
      301 gccacctcca ccagcagctg gaagctgctg aagtcctctt tgtcctgctc tgggggtgtg
      361 ggtggtggcg tgttgaagag tccacctgtg gggcttccag cctcagccct ggtcagcaga
      421 gtaagtgtgc taccaggggt caccaggggc ttgggagact ccagctcccc acgtgggaaa
      481 ggggctgctg gcttttcccc ctggcctgag tgaagcggca tggagcacac agacagggag
      541 agcacattgg tgggggctgg gacagacaca gccagcactg aggggctgct gccacggggg
      601 agggccacat ctgaggcctt acccccgcgg cgggaaatgg taaactgatt agggtctttg
      661 ccatccttcc gaagcatgtc tgggagaagc cgccgccggg cattgatgaa ccagttacat
      721 atctggaatg aatatacaag accgatcaat ttgcttaatc taaaacagct atgggctggg
      781 cacagtggct cacacctgta atcc
//
