LOCUS       sequence-134787-         577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MBP-ccDockerin expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Kowalsky CA, Whitehead TA
  TITLE     Determination of binding affinity upon mutation for type I 
            dockerin-cohesin complexes from Clostridium thermocellum and 
            Clostridium cellulolyticum using deep sequencing.
  JOURNAL   Proteins. 2016 Dec;84(12):1914-1928. doi: 10.1002/prot.25175. Epub 
            2016 Oct 26.
  PUBMED    27699856
REFERENCE   2  (bases 1 to 577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/prot.25175"; journalName: "Proteins"; date: "2016-12"; 
            volume: "84"; issue: "12"; pages: "1914-1928"
COMMENT     Sequence Label: pMAL-ccDockerin
FEATURES             Location/Qualifiers
     source          1..577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="recognition and cleavage site for Genenase(TM) I,
                     a subtilisin BPN' variant engineered for increased 
                     substrate specificity (Carter and Wells, 1987)"
                     /label=Genenase(TM) I site
                     /translation="PGAAHY"
     CDS             249..266
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      305..391
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      483..510
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataacaataa caacaacctc gggccgggtg cggcacacta cgtacatatg gtcattgtat
       61 atggcgatta caacaacgat ggcaatgttg atgcactgga ttttgcaggg ttgaaaaaat
      121 acatcatggc ggcggatcat gcctatgtca agaaccttga tgtaaatctg gacaacgagg
      181 tgaatgcgtt tgacttagcg atcctgaaga aatatctcct cggtatggtt tccaaattgc
      241 ccagcaacca ccaccatcat caccattaag gatccgaatt ccctgcaggt aattaaataa
      301 gcttcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      361 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
      421 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
      481 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt cggtccgttg
      541 tttatttttc taaatacatt caaatatgta tccgctc
//
